Active Cbs Can Be Expressed in Heme-free Systems in the Presence of Metal-substituted Porphyrins or a Chemical Chaperone
نویسندگان
چکیده
ACTIVE CBS CAN BE EXPRESSED IN HEME-FREE SYSTEMS IN THE PRESENCE OF METAL-SUBSTITUTED PORPHYRINS OR A CHEMICAL CHAPERONE. Tomas Majtan, Laishram R. Singh, Liqun Wang, Warren D. Kruger, and Jan P. Kraus* 1 Department of Pediatrics, University of Colorado Denver, 12800 E 19th Ave, Aurora, CO 80045, USA 2 Department of Genomics & Biotechnology, Institute of Molecular Biology SAS, Dubravska cesta 21, Bratislava, 84551, Slovakia 3 Division of Population Science, Fox Chase Cancer Center, 333 Cottman Avenue, Philadelphia, PA 19111, USA Running title: Expression of CBS with heme analogs * Corresponding author: tel: (303) 724-3812, fax: (303) 724-3838, e-mail: [email protected]
منابع مشابه
Enhancement of Solubility and Specific Activity of a Cu/Zn Superoxide Dismutase by Co-expression with a Copper Chaperone in Escherichia coli
Background: Human Cu/Zn superoxide dismutase (hSOD1) is an antioxidant enzyme with potential as a therapeutic agent. However, heterologous expression of hSOD1 has remained an issue due to Cu2+ insufficiency at protein active site, leading to low solubility and enzymatic activity.Objectives:The effect of co-expressed human copper chaperone (hCCS) to enhance the solubility and enzymatic act...
متن کاملPreparation of Symmetrical Tetraphenyl Porphyrin Metal Complexes and Their Spectroscopic Studies
Porphyrins molecules are gaining importance in the present era. Porphyrins are important part of biological molecules like hemoglobin and chlorophyll .Photo system 1 and photo system 2 is important sunlight trap in chloroplast. Electron transport chain is a series of chemical reaction in which energy is formed in the form of ATP (Adenosine triphosphate).The members of electron transport chains ...
متن کاملSpinel ferrites as efficient magnetically reusable nanocatalysts in the solvent-free synthesis of substituted trisphenols
A series of copper and cobalt substituted nanospinel ferrites have been synthesized by the hydrothermal method. These compounds were characterized by FT-IR, XRD, EDX, SEM and VSM techniques. All of the synthesized nanospinel ferrites were found to be highly efficient and magnetically recoverable in the solvent-free synthesis of substituted 2,6-(2-hydroxybenzyl)-phenols (trisphenols). A wide ran...
متن کاملSpinel ferrites as efficient magnetically reusable nanocatalysts in the solvent-free synthesis of substituted trisphenols
A series of copper and cobalt substituted nanospinel ferrites have been synthesized by the hydrothermal method. These compounds were characterized by FT-IR, XRD, EDX, SEM and VSM techniques. All of the synthesized nanospinel ferrites were found to be highly efficient and magnetically recoverable in the solvent-free synthesis of substituted 2,6-(2-hydroxybenzyl)-phenols (trisphenols). A wide ran...
متن کاملSpectroscopic study on the interaction of three water-soluble porphyrins with calf thymus DNA
Porophyrins and their metal derivatives are strong DNA binders with association constant of to . Some of these compounds have been used for the radiations sensitization therapy of cancer and are targeted to interact with cellular DNA. Binding of porphyrins to DNA changes the expectral and other physic chemical characteristic of porphyrins, The mode of binding can be extracted from inspection ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008